Friday, 27 May 2016

NFNF2552 Cosmeceuticals Lab Report





TITLE
Cream Rouge Preparations 

DATE OF EXPERIMENT
24th March2016

OBJECTIVES
1. To formulate cream rouge.
2. To study the factor affecting colour shading in powder.
3. To evaluate the effect of storage condition on the final product.
4. To study the effectiveness of the product.
5. To study the presence of irritancy caused by  the product.

INTRODUCTION
            According to ASEAN Cosmetic Directive, a cosmetic is any substance or preparation intended to be placed in contact with the various external parts of the human body (epidermis, hair system, nails, lips, and external genital organs) or with the teeth and the mucous membranes of the oral cavity with a purpose to clean them, change their appearance and/or correcting body odours and/or protecting them in good condition. According to the U.S. Food and Drug Administration (FDA), cosmetic is defined as any substance intended to be applied to the human body for cleansing, beautifying, promoting attractiveness, or altering the appearance without affecting the body's structure or functions.
             Rouge, commonly knows as blusher, is a cosmetics used to apply colour onto the cheek to add color to the face, give more dimension to the cheekbones, harmonize the face-balance between eye makeup and lipstick and to create subtle changes in the foundation look when lightly dusted over the face.
         
Modern rouge preparations usually come in the forms of liquid suspensions, emulsified cream and lotions, and dry solid forms. Early in ancient Egypt, rouge was prepared in the form of ointments and was also applied on the lips,just as the way lipstick would be used today. Both
men and women wore rouge. The colorants used can either be organic pigments, inorganic pigments, natural dyes or pearlescent pigments.
            Currently, the pressed powder becomes more popular among the consumers due to its desirable matte finishing and long-lasting wear potential. It is applied with a blush brush. This formula works for all skin types especially for oily skin. In modern times, rouge consists of a red-coloured talcum-based powder that is applied with a brush to the cheek.
 
APPARATUS
Evaporating dish, weighing boat, weighing machine, beaker, spatula, pestle and mortar

MATERIALS
Cocoa butter, beeswax, cetyl alcohol, wool fat, liquid paraffin, borax, colourants, water

PROCEDURE
1.      80g of the basic cream formulation is prepared.
2.      The cocoa butter, beeswax, wool fat, cetyl alcohol, liquid paraffin and borax is weighed and is melted on a evaporating dish by using water bath. The mixture is stirred slowly during heating process.
3.      Once the ingredients is melted, the mixture is taken from the heat to cool the mixture.
4.      The water is added to the warm mixture and stirred.
5.      Step 2 to 4 is reapeated to prepare 50g of a cream rouge by adding also the  2% of soluble red colouring agent.
6.      4 other cream rouge is prepared by diluting 2% cream rouge accordingly to obtain various shades of colour with the suggested concentration of colouring agent: 0.125- 2%.
7.      The rouge preparation is kept in the dark and also exposed to sunlight for 2 months.
8.      Skin irritation test is conducted on the cream rouge by putting it on the cheek  for 8 hours.

 
RESULT
1.      Stability testing (storage condition)
Rouge
Colour separation
Change in colour
Viscosity
Contamination
C1
Pigments does not separate out on the surface
Does not change much
Harden
No contamination
Fungal growth and microbial growth are not observed 
C2
Pigments does not separate out on the surface
Does not change much
Harden
No contamination
Fungal growth and microbial growth are not observed 
C3
Pigments does not separate out on the surface
Does not change much
Harden
No contamination
Fungal growth and microbial growth are not observed 
C4
Pigments does not separate out on the surface
Does not change much
Harden
No contamination
Fungal growth and microbial growth are not observed 
C5
Pigments does not separate out on the surface
Does not change much
Harden
No contamination
Fungal growth and microbial growth are not observed 
Table 1: Cream rouges stored in dark place
Rouge
Colour separation
Change in colour
Viscosity
Contamination
C1
Pigments does not separate out on the surface
Lighter than before
(colour intensity decrease)
Soften
No contamination
Fungal growth and microbial growth are not observed 
C2
Pigments does not separate out on the surface
Lighter than before
(colour intensity decrease)
Soften
No contamination
Fungal growth and microbial growth are not observed 
C3
Pigments does not separate out on the surface
Lighter than before
(colour intensity decrease)
Soften
No contamination
Fungal growth and microbial growth are not observed 
C4
Pigments does not separate out on the surface
Lighter than before
(colour intensity decrease)
Soften
No contamination
Fungal growth and microbial growth are not observed 
C5
Pigments does not separate out on the surface
Lighter than before
(colour intensity decrease)
Soften
No contamination
Fungal growth and microbial growth are not observed 
Table 2: Cream rouges placed under the sunlight

2.      Effectiveness

Code
When tested on skin
C1
ü  Highly pigmented
ü  Non-sticky
ü  Mix very well with the skin
ü  Give soothing effect
ü  Give shiny appearance
ü  Skin feels smooth after application
ü  Easy to wash off
ü  Long lasting
C2
ü  Highly pigmented
ü  Less sticky
ü  Mix well with the skin
ü  Give soothing effect
ü  Give shiny appearance
ü  Skin feels smooth after application
ü  Easy to wash off
ü  Long lasting
C3
ü  Less pigmented
ü  Less sticky
ü  Does not mix well with the skin
ü  Give soothing effect
ü  Give shiny appearance
ü  Skin feels smooth after application
ü  Easy to wash off
ü  Last for 5 hours
C4
ü  Less pigmented
ü  Less sticky
ü  Does not mix well with the skin
ü  Give soothing effect
ü  Give shiny appearance
ü  Skin feels smooth after application
ü  Easy to wash off
ü  Last for 4 hours
C5
ü  Very low pigmented
ü  Sticky
ü  Does not mix with the skin
ü  Give soothing effect
ü  Give shiny appearance
ü  Skin feels smooth after application
ü  Easy to wash off
ü  Last for 3 hours


3.      Irritancy

Code
Duration of application
Skin irritancy
C1
8 hours
No irritation occurs
C2
8 hours
No irritation occurs
C3
8 hours
No irritation occurs
C4
8 hours
No irritation occurs
C5
8 hours
No irritation occurs

DISCUSSION
A test is carried out to test the effectiveness of the cream rouge formulated. When cream rouge is applied to skin, all of the cream rouges give soothing effect, smooth and shiny appearance to the skin. This is due to the ingredients used such as cocoa butter, beeswax, cetyl alcohol, wool fat and liquid paraffin which are rich in emollient properties. Emollient is defined as a preparation which gives soothing or softening effect to the skin. The shiny appearance look is due to the oily based formulation which makes the skin looks glowy when light reflection occurs. 
Code C1 and C2 which have high concentration of colorant is highly pigmented when applied to skin while code C3 and C5 is less pigmented. This is due to the variability of the concentration of the colorant added to the formulations. Rouges with high concentration of colorant is highly pigmented when applied to skin while rouges with low concentration of colorant is less pigmented when applied to skin. For code C5, the concentration of colorant is only 0.125% thus it is very low pigmented when applied to skin because the difference in colour of the rouge to the skin is not much significant.
Code C1, is non sticky when applied to skin while C2, C3 and C4 is less sticky, and C5 is sticky when applied to skin. This is because of the presence of wool fat and liquid paraffin which is able to form a film on a skin to trap moisture. However, the degree of stickiness of the rouge may be caused by the concentration of the colorants. The highest content of the colorant stabilize the effect of the protective film over the skin while the lowest content of the colorant makes the protective film effect not stable. Because the function of the wool fat and liquid paraffin is stabilize in rouge C1, it appears non-sticky and because of the instability of the ingredients in rouge C5, it appears sticky when applied to skin.
The rouges are also observed on the ability of it to mix well with the skin. From rouge C1 to C5, the degree of mixing with the skin is decreasing. This may due to the concentration of colorants in the rouge that rouge with high concentration of colorants, i.e. C1 is more stable as compared to rouge with low concentration of colorants, i.e. C5, thus C1 mix well with the skin but C5 does not. Besides, the other factor may be due to human error that the ingredients were not being mixed well during the preparation of formulation. As a result, there are some small granules which is not well mixed in the preparation, thus causing the rouge to not being able to mix well with the skin.
Test for irritation of the skin has also been carried out that the rouges are applied on skin for 8 hours. All of the rouges do not cause any irritation to the skin. Based on the formulation, the potential ingredient to cause irritation is colorants and borax, however due to low content of these ingredients in the formulation, there is no irritation caused. Furthermore, the usage of borax in the formulation acts as preservative has helped to secure the rouges from any microbial action and makes the rouge safe to apply.

1.      Discuss the effect of soluble and insoluble dyes in cream rouges.
A colour additive is any dye, pigment, or other substance that can change the colour appearance of a substance. Dye and pigment show distinct properties; a dye is soluble in its vehicle thus forming a solution whereas a pigment is insoluble in its vehicle that forms a suspension. Dye possesses chemical affinity to the substrate to which it is being applied while a pigment does not have affinity to substrate. There are certain soluble dyes that can be precipitated with a metallic salt to produce a pigment named lake pigment. The purpose of adding dye into rouge is to give the colour effect. There are two types of dye, water soluble and oil soluble; water soluble dye is recommended for colouring soaps, lotions and salts etc while oil soluble dye is used for oil based formulations. In cream rouge, oil soluble dye is used instead of insoluble dye because an insoluble dye gives a rough texture to the cream rouge that is unsuitable to be applied on the cheeks. In addition, colour of insoluble dye is distributed unevenly in a substance, because the colour will sediment at the bottom of oil phase. Oil soluble dyes can dissolve evenly in the oily phase of cream rouge, giving a uniformly coloured product. Good quality cream rouge has even distribution of dye so that the reddening effect is effective.

2.      Show a chart of colour shading for cream rouges at the various colorant concentrations. What you concluded?

Five formulations of cream rouges are produced by varying the concentration of colorant, forming different colours of cream rouges. C1 rouge has the highest colorant concentration and it shows the darkest colour. Colorant concentration is diluted to another four required concentrations to show various colour products. Based on the colour shading chart, the colour density is decreasing from left to right. This concludes that as the concentration of colorant decreases, the colour of the rouge becomes lighter.

3.      How does storage condition affect your preparations?
Cream rouges show different changes when they are stored in dark place and placed under the sunlight. Colour pigment does not separate out on the rouge surface no matter it is stored in dark or under the sunlight. This may indicate that the ingredients in rouges are at stable condition but the small viscosity change shows that the formulations are very unstable as rouges stored in dark are harden than before and rouges placed under the sunlight are soften than before. So there must be a separation of colour pigments out on the surface in which it is not that obvious. Colour is changed after the rouges are placed under the sunlight because light affects the stability of colorant; whereas there is no much colour change in dark. The colour intensity is decreased after it is placed under the sunlight. This is because the colorant disappear as it dissolved in the oily based cream. As for contamination, there is no fungal growth and microbial growth as the rouges do not expose to air, the container is closed. Since the rouges are not exposed to sunlight directly, the observations are not all exactly true especially the contamination. Rouges exposed directly to the sunlight would have a lot of dust on the surface and microbes and fungus might grow. However, by storing the rouges in dark and under sunlight with container closed are the most similar ways of how a rouge user would store it. Normal user would not allow the container to be opened all the time even though the user is not using it. If rouge is exposed to sunlight directly, the colour change will be more distinct.
4.      Explain the function of each ingredient.
Basic cream formulation
Function
Cocoa butter
 Emollient properties, antioxidant, improves skin elasticity, promotes healthy collagen production, reduce stretch marks
Beeswax
Thickening agent, emollient properties
Cetyl alcohol
Emulsion stabilizer and opacifier, adding body and viscosity, emollient properties, carrying agent for other ingredients
Wool fat
Emollient, moisturiser, cushioning and protects the skin, creates a protective film over the skin allowing it to function and breathe naturally, good water hold
Liquid Paraffin
Retain skin moisture, helps keep the skin supple
Borax
Preservative to prevent microbial action
Colorants (Erythrosine)
Give the color to the rouge
Water qs ad
As diluent

5.      Determine the shortcomings of the test procedures. Explain the steps that could be added/modified to overcome that shortcoming and improve the rests.
There are some shortcomings during the preparation, which has caused the rouge after formulation to be a bit less viscous as normal rouge. First of all, the ingredients used may have contaminated, therefore gloves are worn when handling the ingredients, apparatus are ensured to be clean from dust and the container of the ingredients are always covered after use. Next, the ingredients were not well mixed during the preparation in the pestle with mortar thus causing the resulting rouge to have some small granules. After that, the temperature of the water bath used to heat the ingredients at the beginning may not be suitable for the formulation, thus causing the rouge to be less viscous. Then, parallax error may have occurred during the experiment which contributes to the inaccurate results of the experiment. The eye should be parallel to the scale whenever a measurement is taken.

CONCLUSION
             Overall, the formulation of the cream rouge was a success. However, there are several errors that affect the texture of the cream rouge. The formulation does not cause irritancy during 8 hour of application. The effectiveness of the cream rouges formulated (pigmentation formed, ability to mix well and stickiness) is overally good. The concentration of the colorant affects the color shading of cream rouge. As the concentration of colorant decreases, the colour of the rouge becomes lighter. Colour is changed after the rouges are placed under the sunlight because light affects the stability of colorant; whereas there is no much colour change in dark. The cream rouges are best to be placed in dark condition as light will lower the stability of colorant.

REFERENCES

 

TITLE
Deodorant Preparations

DATE OF EXPERIMENT
27th April 2016

INTRODUCTION
            Perspiration is a thermoregulation process that cools down our body and maintains equilibrium in our system. Perspiration itself does not cause body odour. Body odour is caused by naturally occurring bacteria feed on sweat secretions from the apocrine gland and release waste products.
            Deodorant is a substance that contain antimicrobial agent that can prevent body odour when applied to the body. There are a few strategies of deodorant formulations to reduce underarm odour such as antimicrobial active-containing deodorants, antiperspirant active-containing deodorants, odour-masking deodorants, odour-neutralizing deodorants, odour-quenching deodorants and esterase inhibitors. Anti-perspirants differ from deodorants in which it contains ingredients that control sweat and prevent sweat from reaching the skin surface, thus reducing the bacteria that cause body odour.

OBJECTIVES
1. To formulate a deodorant.
2. To evaluate the effectiveness of the deodorant and identify if there any irritation 
    cause by the product.
3. To evaluate the effect of the storage condition of the final product.


APPARATUS
Water bath, evaporating dish, glass rod, container.


MATERIALS
Beeswax, cocoa butter, coconut oil, silicone oil, vitamin E, corn starch, essential 
oil/perfumes


PROCEDURES
1. The cocoa butter and beeswax was heat until they melted. Coconut, silicone and
    vitamin E oil were added.
2. When all the oils and fats have melted, the essential oil was then added into the mixture.
3. The corn starch was mixed by using hand mixer until homogenous.
4. The molten mixture was poured into the container and was allowed to cool until it hardens.

 
INGREDIENTS AND CALCULATION:

INGREDIENTS
PERCENTAGE (%)
WEIGHT(g) (for 20g of products)
Beeswax
20
4g
Cocoa butter
30
6g
Coconut oil
30
6g
Silicone oil
3
0.6g
Vitamin E oil
2
0.4g
Corn starch
12
2.4g
*Essential oil/perfumes
Not more than 3
0.6g

* Essential oils/ perfume used:
A : Topaz (0.6g)
B : Rose (0.6g)
C : Citrus (0.6g)
D : Apple Blossom (0.6g)
E : Flora (0.6g)

EVALUATION OF PRODUCTS
1. Each member of our group took their turn to test the products.
2. A simple questionnaire that will allow each person to compare the effectiveness of each product and whether the fragrance incorporated is irritating to the skin was devise.
3. In the morning, a shower was taken for the test and was dried by a towel. The underarm that will function as the control was determined. Any preparation was not applied on this underarm. The same underarm was used as the control throughout the study.
4. On the other underarm, not more than 1g of our product was applied.
5. Exactly after 9 hours of normal daily activities, the questionnaire was filled.
STABILITY TESTING
1. The remaining products were placed in room temperature and sunny position.
2. After 4 weeks, the product was evaluated.



STABILITY TESTING
Deodorant
Storage Condition
Colour Intensity
Room temperature
Sunny
Topaz
More soften
Less soften
No change
Rose
More soften
Less soften
No change
Flora
More soften
Less soften
No change
Apple Blossom
More soften
Less soften
No change
Citrus
More soften
Less soften
No change

DISCUSSION
For this practical session, the products prepared were roll on deodorant.  By definition, a deodorant is a substance applied to the body designed to reduce body odor majorly caused by the bacterial breakdown of perspiration in armpits, feet, and other areas of the body. It is often less irritating than an anti-perspirant.
Deodorants are often alcohol-based. Alcohol initially stimulates sweating but it also temporarily kills bacteria. Beeswax is one of the ingredients used in making the deodorant. Beeswax helps to keep an emulsion from separating into its oil and liquid components. Beeswax also increase the thickness of the lipid (oil) portion of solid and stick-like products, giving them structure, allowing for a smooth application, and keeping them solid. Next is cocoa butter. Cocoa butter is a rich emollient, it leaves the skin buttery soft. It contains fatty acids which help to retain moisture and build elasticity and also has properties which can help ease dermatitis or rashes. Coconut oil functions as an anti-caking agent, surfactants, and as skin conditioning agents. Silicone oil used as one of the ingredients in this experiment has a low surface tension properties combined with viscosity, can make other components of cosmetics easily spread into a thin film on the skin, and no sticky feeling. Vitamin E is said to have anti-inflammatory and analgesic (pain relieving) properties. Studies also suggest that topical application of vitamin E can decrease skin roughness, dryness and fine lines, which explains the functions of vitamin E in the deodorant. While for corn starch, it acts as skin protectant (absorb water, surfactants and viscosity increasing agents. Finally, perfumes and fragrances are used in deodorants and antiperspirants in order to mask body odour and provide a feeling of freshness to the user. In addition to fragrance that they impart, fragrances/essential oil also acts as antimicrobial agents.
A simple questionnaire is constructed to evaluate the functionality of the prepared deodorant based on the factors that should be considered upon designing one. The factors include high efficacy in masking body odour, long-term deodorization, no irritation potential, the good skin feeling of the product and the good selection of fragrance. The response from the respondents for the corresponding items in the survey is measured with Likert scale ranging from 1 (strongly disagree) to 5 (strongly agree).
Based on the data obtained, for the five deodorant of different type of fragrances, almost all of the respondents give positive response which shows that they feel comfortable after applying the deodorant, do not experience skin irritancy, feel satisfied with its ability to mask body odour, feel pleasant with the scent and think that the deodorant is effective in long term deodorization. Hence, the feedback given proves that the deodorants prepared are highly effective. After 4 weeks, the colour intensity of the 5 deodorants remains unchanged. However, the different storage conditions affect the nature of the deodorant. In sunny condition, the deodorants are less soften than in room temperature condition. This may due to the difference in the humidity of air for both conditions.
 
CONCLUSION
           
            Overall, the formulation is success as the formulated product can perform both deodorant and anti-perspirants properties where body odour is masked and sweating is controlled.

            Based on the evaluation tests carried out, it can be concluded that the products are stable and do not cause any skin irritation.

REFERENCES
 
hp://www.anperspirantsinfo.com/en/anperspirants-and-deodorants/about-anperspirants-and-
deodorants.aspx
hp://www.cosmecsinfo.org/ingredient
http://draxe.com/cocoa-butter/

http://www.paulaschoice.com/cosmetic-ingredient-dictionary/definition

No comments:

Post a Comment